• 专利标题:   Detecting dopamine concentration in sample comprises e.g. taking sucrose as raw material to obtain carbon dot solution, modifying glassy carbon electrode with graphene oxide and carbon dot solution to obtain modified glassy carbon electrode.
  • 专利号:   CN107525837-A, CN107525837-B
  • 发明人:   XIAO Q, HUANG S, LI J
  • 专利权人:   UNIV GUANGXI TEACHERS EDUCATION, UNIV GUANGXI TEACHERS EDUCATION
  • 国际专利分类:   G01N027/327, G01N027/48
  • 专利详细信息:   CN107525837-A 29 Dec 2017 G01N-027/327 201816 Pages: 12 Chinese
  • 申请详细信息:   CN107525837-A CN10765619 30 Aug 2017
  • 优先权号:   CN10765619

▎ 摘  要

NOVELTY - Detecting dopamine concentration in sample comprises e.g. (i) taking sucrose as main raw material to obtain carbon dot solution and storing, (ii) modifying glassy carbon electrode with a solution of graphene oxide and carbon dot solution to obtain modified glassy carbon electrode and (iii) preparing phosphate-buffered saline containing different concentrations of dopamine, using modified glassy carbon electrode as working electrode forming a three-electrode system by cyclic voltammetry in order to detect more than two kinds of buffer solution and recording oxidation peak current value. USE - The method is useful for detecting dopamine concentration in sample. ADVANTAGE - The method: is simple and convenient; has high sensitivity and low detection limit; and can rapidly detects dopamine concentration in actual sample. DETAILED DESCRIPTION - Detecting dopamine concentration in sample comprises (i) taking sucrose as main raw material to obtain 5 mg/ml carbon dot solution and storing at 4 degrees C, (ii) modifying glassy carbon electrode with a solution of the graphene oxide and the carbon dot solution obtained in the step (i) at a concentration of 3 mg/ml to obtain modified glassy carbon electrode, (iii) preparing phosphate-buffered saline containing different concentrations of dopamine, using modified glassy carbon electrode as working electrode forming a three-electrode system by cyclic voltammetry in order to detect more than two kinds of buffer solution, recording oxidation peak current value of multiple buffer solution, establishing relation equation of the dopamine concentration and oxidation peak current value in three-electrode system, and (iv) adding dopamine of the unknown concentration into the phosphate-buffered saline solution to be tested buffer solution, using three-electrode system to test buffer solution to be tested, recording corresponding oxidation peak current value to-be-tested buffer solution and oxidation peak current value into the third step in the relationship equation, and solving the unknown concentration of dopamine concentration.