▎ 摘　　要

NOVELTY - Graphene field-effect transistor-based biosensor comprises silicon substrate that forms infrastructure of the biosensor, reduced graphene oxide layer and silicon oxide layer that enables the gold/titanium electrodes to be placed on the silicon substrate and to prevent unwanted electrical transmissions. The reduced graphene oxide layer that enables lung cancer-specific biomarkers (miRNA) to be attached to the structure without the need for an additional chemical substance, where the reduced structure of graphene improves its electrical conductivity quality. The gold/titanium electrodes enable to receive signals and attach to the silicon oxide layer. USE - The graphene field-effect transistor-based biosensor is useful in early detection of lung cancer. ADVANTAGE - The graphene field-effect transistor-based biosensor detects lung cancer at early stage with high selectivity, high sensitivity and high accuracy and measures the level of biomarkers in the blood with high precision and high accuracy without the need for procedures to be applied in the hospital. The recovery rate of patients with early diagnosed lung cancer is quite high. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method for immobilizing graphene field-effect transistor-based biosensor on the reduced structure of miRNA, which involves washing the reduced graphene structure before miRNA-1268b with 1x phosphate buffered saline (PBS) and then with deionized water, after washing, placing on the heating plate, completely evaporating the water remaining on the structure, after the structure dries, dripping 5 μ l onto the reduced graphene oxide structure of miRNA-1268, which is a lung cancer-specific biomarker without using any binding material, and for the incubation of the miRNA1268b structure, keeping the structure on the heating plate for 30 minutes.