• 专利标题:   Method for preparing silver-graphene nanocomposite-based peroxide enzyme sensors, involves weighing graphene oxide into water, dissolving and centrifuging, followed by putting in electrodeposition solution and performing electrodeposition.
  • 专利号:   CN106383158-A, CN106383158-B
  • 发明人:   LI S, WEI S, CHENG C
  • 专利权人:   UNIV ANYANG NORMAL, UNIV ANYANG NORMAL
  • 国际专利分类:   G01N027/30
  • 专利详细信息:   CN106383158-A 08 Feb 2017 G01N-027/30 201720 Pages: 10 Chinese
  • 申请详细信息:   CN106383158-A CN10989211 10 Nov 2016
  • 优先权号:   CN10989211

▎ 摘  要

NOVELTY - A silver-graphene nanocomposite-based peroxide enzyme sensor preparing method involves weighing graphene oxide into water, dissolving and centrifuging to obtain a brownish graphene solution. Electrodeposition solution is prepared. The graphene solution is applied on the surface of a pretreated glassy carbon electrode, followed by performing one-step electrodeposition on a electrochemical workstation using a three-electrode system, putting in electrodeposition solution, and performing constant potential deposition of -0.75V and -0.55V for 1-3 minutes to obtain the finished product. USE - Method for preparing silver-graphene nanocomposite-based peroxide enzyme sensors. ADVANTAGE - The method enables preparing silver-graphene nanocomposite-based peroxide enzyme sensors with high sensitive and selectivity detection. DETAILED DESCRIPTION - A silver-graphene nanocomposite-based peroxide enzyme sensor preparing method involves weighing 5-10 mg graphene oxide into 5-10 ml water, ultrasonic dissolving for 2-3 hours to obtain an oxidized graphene dispersion. The oxidized graphene dispersion is centrifuged to remove undissolved graphene oxide to obtain a brownish graphene solution. Electrodeposition solution is prepared by mixing 1-10 mmol/l silver nitrate and 0.05-0.5 mol/l potassium nitrate. The graphene solution of 10-15 mu l is applied dropwise on the surface of a pretreated glassy carbon electrode, allowed to stand at room temperature to dry the graphene modified glassy carbon electrode, followed by performing one-step electrodeposition on a electrochemical workstation using a three-electrode system comprising graphene oxide modified glassy carbon electrode as the working electrode, platinum wire electrode as counter electrode and saturated calomel electrode as reference electrode, putting in electrodeposition solution, and performing constant potential deposition of -0.75V and -0.55V for 1-3 minutes to obtain the finished product.