▎ 摘　　要

NOVELTY - Developing quartz crystal microbalance (QCM) sensor using various nano particle/thin films to enhance the performance sensitivity of the biosensor. The biosensing device is focusing on immunosensing. The thin film used is reduced graphene oxide/carbon nanotube (CNT). A process of low cost synthesis of graphene/CNT proposed using a general laboratory level approach-electrochemical exfoliation. The graphene is a multilayer graphene. The method of producing QCM sensor using thin film also involves an end-to-end signal conditioning circuit. The sensing is based on dissipation technique which will provide a label-free quantitative method. The label-free quantitative method includes resonance frequency analysis technique. The measurement using the dissipation technique claiming as QCM with dissipation monitoring sensor. USE - The method is useful for developing QCM sensor or producing QCM sensor (claimed), which is useful for: biomolecular interactions e.g. antigen-antibody reaction, pathogen detection, cell adhesion, adsorption, characterization of adsorbed proteins, DNA and RNA interactions with complementary strands, and detection of bacteria and viruses; measuring the change in the system frequency due to the change in the system characteristics; and measuring the rate of energy dissipation in the surrounding medium and detection. ADVANTAGE - The method: allows a direct monitoring of change in some physical parameters with ease of use; utilizes immunosensors that are simple, provides fast response, and are low cost and highly sensitive; and develops a label-free, simple, cost effective, quantitative nano-layered immunosensing system with enhanced sensitivity.