▎ 摘　　要

NOVELTY - An antibacterial collagen-based bone repair material comprises zinc oxide/hydroxyapatite whiskers, type I collagen, polyvinyl alcohol, cross-linking agent, graphene oxide, polydopamine, polypyrrole doped with sulfosalicylic acid self-assembled layer and bone morphogenetic protein-2 immobilized on the self-assembled layer. USE - Antibacterial collagen-based bone repair material. ADVANTAGE - The bone repair material has excellent biocompatibility and antibacterial effect on the surface and inside, which can avoid inflammation and infection during bone transplantation, and uses non-toxic and harmless raw material. The self-assemble of polypyrrole polydopamine coating on the surface of the bone repair material can firmly load and stabilize the release of bone morphogenetic protein-2 and improve the utilization rate of bone morphogenetic protein 2, at same time, avoids the complications caused by excessive concentration of bone morphogenetic protein-2. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for preparation of the antibacterial collagen-based bone repair material, which involves dissolving type I collagen in glacial acetic acid solution, adding zinc oxide/hydroxyapatite whiskers, stirring, adding polyvinyl alcohol solution and stirring into mixed solution, pouring mixed solution into mod, freezing, vacuum-drying, placing in cross-linking agent solution for 24 hours, taking out material, immersing into disodium hydrogen phosphate solution for 2-3 hours, taking out material and immersing in sodium chloride solution for 18-30 hours where sodium chloride solution is replaced regularly and shaken with large amount of distilled water to wash until sodium chloride is completely precipitated, freeze-drying to obtain bone repair material, immersing bone repair material in polyethyleneimine solution for amination modification, taking out and washing, drying and then immersing in hydrochloric acid solution, washing, drying to obtain triamine-modified bone repair material, immersing the triamine-modified bone repair material in the graphene oxide dispersion liquid to self-assemble a layer of graphene oxide on the surface, taking out, washing with water, placing in the three-electrode system of calcium nitrate solution electrolyte with dopant sulfosalicylic acid, pyrrole monomer and dopamine monomer, performing polypyrrole-polydopamine self-assembly under the alternating effect of two pulse voltages where the number of cycles controls the number of layers of polypyrrole-polydopamine, washing the bone repair material with deionized water, vacuum drying, immersing in the bone morphogenetic protein-2 solution to immobilize bone morphogenetic protein, taking out, and drying at 37 degrees C in oven.