▎ 摘　　要

NOVELTY - Method of detecting histamine based on fluorescence resonance energy transfer biosensor with magnetic nanoparticle separation involves immobilizing the histamine antibody on the surface-aminated graphene quantum dots, preparing gold nanoparticles, modifying the surface of gold nanoparticles, linking the magnetic nanoparticles having surface carboxy groups with the histamine antibody, enriching the histamine using the magnetic nanoparticles, adding the magnetic separation to the mixed solution of graphene quantum dots and gold nanoparticles and measuring the fluorescence intensity. USE - The method is useful for detecting histamine (claimed). ADVANTAGE - The detection method is rapid and sensitive and has detection limit of 24 pM. DETAILED DESCRIPTION - Method of detecting histamine based on fluorescence resonance energy transfer biosensor with magnetic nanoparticle separation involves (a) immobilizing the histamine antibody on the surface-aminated graphene quantum dots using glutaraldehyde and storing at 0-4 degrees C, (b) preparing gold nanoparticles by sodium citrate reduction of high chloroauric acid, modifying the surface of gold nanoparticles using histamine antibody and storing modified gold nanoparticles at 0-4 degrees C, (c) linking the magnetic nanoparticles having surface carboxy groups with histamine antibody by activation with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and (d) enriching the histamine using the magnetic nanoparticles obtained in step (c), adding the magnetic separation to the mixed solution of graphene quantum dots and gold nanoparticles, incubating the mixture for 20-40 minutes and measuring the fluorescence intensity.