▎ 摘　　要

NOVELTY - Detecting uric acid with uric acid oxidase-loaded ferric tetroxide composite nano-enzyme, involves obtaining uric acid working curve by mixing a solution A of 100 microl uric acid, solution B of 100 microl ferric tetroxide-graphene-hemin or guanosine-5'-monophosphate nanomaterial or solution C of 100 microl tetramethylbenzidine, adding 1.5 ml phosphate-buffered saline buffer having a pH of 4, incubating the mixture at 37degreesC for 15 minutes, measuring the absorbance at 652 nm wavelength, drawing a standard curve, and obtaining the regression equation. USE - Method for detecting uric acid in blood, urine and saliva for diagnosis and treatment of diseases, such as gout, arthritis, kidney stone and hyperuricemia. ADVANTAGE - The method enables rapidly detecting uric acid with high recovery rate of 96.3-102.9%, simple operation and high sensitivity and specificity DETAILED DESCRIPTION - Detecting uric acid with uric acid oxidase-loaded ferric tetroxide composite nano-enzyme, involves obtaining uric acid working curve by mixing a solution A of 100 microl uric acid, solution B of 100 microl magnetite- graphene-hemin or guanosine-5'-monophosphate nanomaterial or solution C of 100 microl tetramethylbenzidine, adding 1.5 ml phosphate-buffered saline buffer having a pH of 4, incubating the mixture at 37degreesC for 15 minutes, measuring the absorbance at 652 nm wavelength, drawing a standard curve, and obtaining the regression equation, where the uric acid in blood is determined by adding 5 ml fresh blood into a centrifuge for centrifugation, separating the supernatant, adding 0.1 ml sodium hydroxide solution with 10 wt.%/vol concentration and 1 ml of 5 wt.%/vol zinc sulfate solution, mixing, centrifuging, separating 100 microl of the supernatant, adding 100 microl of 1 mg/ml magnetite-graphene-hemin or guanosine-5'-monophosphate nanomaterial, 100 microl of 10 mmol/l tetramethylbenzidine and phosphate-buffered saline buffer, incubating at 37degreesC for 15 minutes, measuring the absorbance at a wavelength of 652 nm, and measuring the uric acid content.