▎ 摘　　要

NOVELTY - Electrochemical biosensor comprises reduced and oxidized graphene-gold nanocomposite material, biological nanocapsule, and leptin antibody modified on surface of electrode USE - The biosensor is useful in leptin electrochemical biosensor for detecting content of leptin in diet induced obesity symptoms (claimed). ADVANTAGE - The biosensor has high selectivity, sensitivity and anti-interference ability; and provides detection range of 0.001-1000 pg/ml with detection limit of 0.00087 pg/ml. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: preparing the electrochemical biosensor, comprising placing the reduced and oxidized graphene-gold nanocomposite material on the surface of the electrode, drying, placing biological nanocapsule and incubating, placing leptin antibody on the surface of the electrode, and incubating. use of electrochemical biosensor in detecting leptin. leptin electrochemical biosensor, comprising electrochemical biosensor.