▎ 摘　　要

NOVELTY - Preparing copper nanowire-molybdenum disulfide-graphene complex comprises e.g. (i) dissolving sodium hydroxide in N, N-dimethylformamide (DMF) solution, dispersing in DMF, stirring, adding the above 1-aminopropyl-3-methylimidazoliumbromide solution, heating, and cooling to obtain graphene suspension, (ii) adding sodium molybdate dihydrate and L-cysteine to the graphene suspension, and reacting to obtain a black product, washing by centrifugation to obtain molybdenum disulfide-graphene complex suspension, (iii) dissolving copper salt dihydrate in a conical flask of 10 ml secondary water, adding octadecylamine, glucose, and glycine during stirring, and heating, transferring the mixed solution, heating reaction kettle to obtain a red-brown product, centrifuging, and filtering with ethanol, (iv) adding to the molybdenum disulfide-graphene complex suspension prepared in the step (ii), ultrasonically dispersing, and stirring the copper nanowire solution. USE - The copper nanowire-molybdenum disulfide-graphene complex is useful in detection of troponin (claimed). ADVANTAGE - The complex: has excellent conductive effect, large specific surface area and catalytic performance; improves selectivity and sensitivity; and provides a simple and fast detection method for troponin with high sensitivity and excellent selectivity. DETAILED DESCRIPTION - Preparing copper nanowire-molybdenum disulfide-graphene complex comprises (i) dissolving sodium hydroxide in N, N-dimethylformamide (DMF) solution containing ionic liquid 1-aminopropyl-3-methylimidazoliumbromide, homogenizing graphene oxide, dispersing in DMF, stirring for 25-35 minutes, adding the above 1-aminopropyl-3-methylimidazoliumbromide solution during stirring, adding to water bath at 75-85 degrees C for 11-15 hours, adding hydrazine hydrate and continue heating for 1-2 hours, and cooling to room temperature to obtain graphene suspension, where the mass ratio of sodium hydroxide, 1-aminopropyl-3-methylimidazoliumbromide, graphene oxide, hydrazine hydrate and DMF is 6-8:1:2:5-10:9000-10000, (ii) adding sodium molybdate dihydrate and L-cysteine (L-Cys) to the graphene suspension prepared in the step (i), and transferring the mixed solution obtained by stirring to the reaction kettle, and reacting at 180-190 degrees C for 35-37 hours to obtain a black product, washing by centrifugation to obtain molybdenum disulfide-graphene complex suspension, where the sodium molybdate dihydrate, L-cysteine, graphene suspension, and DMF is 9-11:19-21:2:36000-40000, (iii) dissolving copper salt dihydrate in a conical flask of 10 ml secondary water, adding octadecylamine, glucose, and glycine during stirring, and heating at 45-55 degrees C for 5-15 minutes, transferring the mixed solution, heating reaction kettle at 100-105 degrees C for 5-10 hours to obtain a red-brown product, centrifuging the product with ethanol/water (1/1) and ethanol solvent at 7000-9000 revolutions/minute (rpm) for 8-15 minutes, and filtering with ethanol, and adjusting the volume with ethanol to obtain copper nanowire solution, where the mass ratio of the copper salt dihydrate, octadecylamine, glucose, glycine and ethanol is 38:170-190:45-55:55-65:35000-45000, and (iv) adding the copper nanowire solution to the molybdenum disulfide-graphene complex suspension prepared in the step (ii), ultrasonically dispersing for 15-25 minutes, and stirring the copper nanowire solution obtained for 3.5-4.5 hours, where the copper nano wire and molybdenum disulfide-graphene complex according to the weight ratio of 0.05-0.06:6-10. INDEPENDENT CLAIMS are also included for: (1) preparing a troponin electrochemical aptamer sensor comprising (a) taking a polished glassy carbon electrode (GCE), applying 5-10 mu l of the copper nanowire-molybdenum disulfide-graphene complex solution prepared on its surface, and drying it at room temperature to obtain copper nanowire-molybdenum disulfide-graphene complex-modified glassy carbon electrode, and (b) taking 5-10 mu l of troponin aptamer and amino-modified aptamer complementary strand to obtain 1.25 mu M double-stranded DNA through base complementary pairing, dropping onto the surface of copper nanowire-molybdenum disulfide-graphene complex/glassy carbon electrodein the step (a), reacting at 4 degrees C for 11-13 hours; and (2) detecting troponin comprising (I) preparing many concentrations of troponin standard solutions, (II) using the prepared troponin electrochemical aptamer sensor as a working electrode, a saturated calomel electrode as a reference electrode, and a platinum column electrode as a counter electrode, adding 5 mM ferrocyanide solution containing 0.1 M potassium chloride as the electrolyte to form a three-electrode system, adding the first concentration of troponin standard solution prepared in the step (I) to the three-electrode system and incubate at room temperature for 20-40 minutes, measuring the electrochemical signal of the troponin standard solution by electrochemical impedance (EIS), the EIS signals of the remaining concentrations are sequentially measured, a linear relationship between the resistance value obtained by the R (C (RW)) fitting and the negative logarithm of the concentration of the troponin standard solution is used to establish a standard working curve.