▎ 摘　　要

NOVELTY - Graphene sensor has a substrate. The substrate is provided with a source electrode, a drain electrode and graphene conductive layer (23) bridging the source electrode and the drain electrode. A sample cell has a first mounting cavity and a second mounting cavity. The sample cell is arranged the substrate to cooperate with the substrate to define a connecting cavity connected with the first mounting cavity and the second mounting cavity. The second mounting cavity is utilized for mounting a gate. The small chamber (40) is provided with a culture cavity for containing the to-be-detected object and is installed on the first mounting cavity. A portion of the conductive layer graphene is located in the connecting cavity and opposite to the second mounting cavity. The small chamber is provided with a micro-hole connected with the culture cavity and the first mounting cavity. USE - Graphene sensor for real-time in situ detection for use in real-time in situ detection system (claimed). ADVANTAGE - The sensor detects cell while culturing, realizes real-time in-situ detection, does not change the properties of detection molecules, is easy to operate, shorts detection time, and has high sensitivity and stability. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a real-time in situ detection system, which comprises a probe station and a graphene sensor for real-time in situ detection, where the graphene sensor is placed on the probe table; (2) a manufacturing method for the graphene, which involves: (a) obtaining the substrate, the source electrode, the drain electrode, the grid electrode, the graphene cell and the small chamber; (b) attaching the source electrode on the substrate, the drain electrode and the graphene film, making the film graphene the source electrode and the drain electrode; (c) placing the sample cell on the substrate to define the communication cavity (34) communicating the first mounting cavity and the second mounting cavity; and (d) performing albumin antibody functional treatment to the thin film graphene to obtain the conductive layer graphene; and (3) a real-time in situ detection method of hepatocellular differentiation, which involves: (a) obtaining hepatocyte; (b) paving the mixture of the liver cell and substrate glue on the permeable membrane of the small chamber, and adding culture liquid to stabilize; (c) mounting the small chamber to the first mounting cavity, and adding differentiation liquid to culture; (d) placing the graphene sensor on the probe station access detection circuit, and inserting the gate into the second mounting cavity; and (e) recording the I-V curve of the conductive layer graphene. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic view of graphene sensor. 23Graphene conductive layer 32Second installation cavity 33Groove 34Communication cavity 40Small chamber