▎ 摘　　要

NOVELTY - Developing an electroanalytical biosensor for acetylcholine detection, comprises: vector scanning of a polyimide sheet (201) making a laser-induced graphene (LIG); treating the LIG surface with sodium hydroxide solution for incorporation of myriad of oxygen groups; immobilizing acetylcholinesterase enzyme onto the sodium hydroxide treated LIG surface; binding of acetylcholine with the immobilized acetylcholinesterase enzyme onto anodized LIG surface; performing an electrochemical transduction; and displaying result, where the vector scanning of polyimide sheet is by using 0.1-100 W carbon dioxide laser, and treating the LIG surface is by using 0.1-1 M sodium hydroxide solution. USE - The method is useful for developing an electroanalytical biosensor for acetylcholine detection (claimed), which is useful for mitigating the issues of poor sensor performance by adopting anodized LIG surfaces. ADVANTAGE - The method designs and develops an electroanalytical biosensor for selective and sensitive detection of acetylcholine, which: can provide an increased overall shelf-life digital and of which operation can be easily integrated towards device applications for early monitoring of the neurotransmitter-acetylcholine i.e. necessary to prevent life-threatening circumstances; is portable, handheld, lightweight, cost effective and highly sensitive; utilizes anodization that ensures strong binding of the receptor acetylcholinesterase enzyme onto the LIG, which can then efficiently catalyze the hydrolysis of acetylcholine into choline and acetate and can be easily calibrated voltammetrically (electrochemical) to specific concentrations of the neurotransmitter; and exhibits ultra-low limit of detection (0.041 pM) and sensitivity (0.11 mA/nM/cm2) within a wide concentration of 1-100 pM. DESCRIPTION OF DRAWING(S) - The figure shows the schematic view of components and process of vector scanning of the polyamide sheet using laser beam. Polyimide sheet (201) Vector-scanning laser probe (202) Laser beam (203)