▎ 摘　　要

NOVELTY - Exosome detecting micro-flow control chip based on graphene oxide quenching nucleic acid aptamer comprises upper layer, lower layer and bottom surface layer orderly connected in series. The upper layer is sample liquid layer. The lower layer is nucleic acid aptamer liquid layer quenched by graphene oxide. The bottom surface is blank glass bottom plate. The sample inlet and reaction tank are arranged between sample inlet and reaction tank for communication. The nucleic acid aptamer liquid layer is located at most upstream of whole oxidized graphene quenching. The nucleic acid aptamer sample inlet (1) is arranged on graphene oxide quenching and reaction tank (3) for communicating two. The reaction tank is arranged between nucleic acid aptamer feeding channel region of graphene oxide quenching and sample feeding channel region (2) to be tested, vertically through whole chip, for communicating two. USE - Exosome detecting micro-flow control chip based on graphene oxide quenching nucleic acid aptamer used for detecting exosomes in body fluids such as blood, urine and saliva, and culture media (claimed). ADVANTAGE - The exosome detecting micro-flow control chip based on graphene oxide quenching nucleic acid aptamer detects multiple markers of multiple samples at same time, improves exosome, simplifies manual operation, and obtains good exosome detecting effect. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for using microfluidic chip for detecting exosomes based on graphene oxide quenching nucleic acid aptamer, which involves: (A) adding 5-20 nanomolar nucleic acid aptamer of relevant protein to be detected and 0.02-0.2 mg/ml graphene oxide aqueous solution to buffer (Tris-hydrochloric acid (HCl) buffer or phosphate buffer salt solution), and reacting at room temperature for 5-20 minutes to completely quench fluorescence of aptamer; (B) using syringe pump to add graphene oxide-quenched nucleic acid aptamer probe into reaction cell, where volume of each reaction cell is 10-30 microliter; (C) using syringe pump to add sample to 0.1-0.3 microliter reaction tank; (D) reacting at room temperature, and avoiding light for 20-40 minutes; and (E) detecting fluorescence value of system. DESCRIPTION OF DRAWING(S) - The drawing shows schematic view of exosome detecting micro-flow control chip based on graphene oxide quenching nucleic acid aptamer. Sample inlet (1) Sample feeding channel region (2) Reaction tank (3)