• 专利标题:   Nanopore sequencing device for determining sequence of biopolymer e.g. polynucleotide or polypeptide, has micro-electro-mechanical system that generates bubble sufficient to interrupt ionic current flow between cis and trans electrodes.
  • 专利号:   WO2023055652-A1, US2023112203-A1, TW202317993-A
  • 发明人:   BOYANOV B, CIESLA C, FLANNERY A, KREINDL G, MUSA R S, SEGALE D
  • 专利权人:   ILLUMINA INC, ILLUMINA SINGAPORE PTE LTD, ILLUMINA SINGAPORE PTE LTD, ILLUMINA INC
  • 国际专利分类:   G01N027/414, G01N033/487, B01L003/00, C12Q001/6869, G01N033/68, B82Y035/00
  • 专利详细信息:   WO2023055652-A1 06 Apr 2023 G01N-033/487 202334 Pages: 77 English
  • 申请详细信息:   WO2023055652-A1 WOUS044432 22 Sep 2022
  • 优先权号:   US261937P, US934175

▎ 摘  要

NOVELTY - The device has an array of nanopore cells, where each of the nanopore cell comprises a cis well, and a trans well. A membrane support is in between the cis and trans wells. a bubble generating electrode is arranged in the trans well, where the device comprises a wafer including many sequencing channels configured to allow a biopolymer to translocate substantially horizontally or substantially vertically with respect to a surface of the wafer. The membrane support is formed of lipid, silicon, graphene, a solid-state material, a synthetic material, or a biomimetic equivalent of lipid. USE - Nanopore sequencing device for determining a sequence of a biopolymer, such as a polynucleotide or polypeptide. ADVANTAGE - The device mitigates undesirable side effects that occurs when operating a nanopore sequencing array, which prevents a few malfunctioning or failed nanopore sensor devices from affecting the operation of the entire sequencing array, and prevents the consumption of redox reagents resulting from a large uncontrolled current passing through a malfunctioning, failed, or malfunctioning sensor within an array. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a method for isolating a unit in an array for polynucleotide sequencing. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic view of a nanopore sequencing device. 600Nanopore unit cell 615Middle well 620Electrolyte 623Nanopore 624Membrane