▎ 摘　　要

NOVELTY - Preparing platinum/gold modified boron/sulfur/nitrogen doped reducing oxidation graphene (Pt/Au-BSN-rGO) involves adding the graphite powder and sodium nitrate into sulfuric acid, stirring for 1-2 hours, adding potassium permanganate, stirring for 1-2 hours, adding water, continuously stirring for 1-2 hours, heating and refluxing for 1-3 hours, adding hydrogen peroxide to stop reaction, washing, freeze-drying, grinding to obtain the oxidation graphene by mixing the oxidation graphene L-cysteine and boric acid obtained in the step one, adding deionized water, ultrasonic, adjusting pH to 8-9, transferring to the reaction vessel, hydrothermal reaction, cooling, washing, freeze-drying to obtain boron/sulfur/nitrogen-doped reducing oxidized graphene powder, dispersing the boron/sulfur/nitrogen-doped reducing oxidized graphene powder (BSN-rGO) obtained in step two in water, ultrasonic for 1-2 hours to form suspension, adding chloroauric acid (HAuCl4) solution. USE - Method for preparing platinum/gold modified boron/sulfur/nitrogen doped reducing oxidation graphene for use in electrochemical immune sensor (claimed). ADVANTAGE - The method effectively amplifies the current response signal, enhances the sensitivity of the electrochemical immune sensor, and has wide linear range, high sensitivity and low detection limit. DETAILED DESCRIPTION - Preparing platinum/gold modified boron/sulfur/nitrogen doped reducing oxidation graphene (Pt/Au-BSN-rGO) involves adding the graphite powder and sodium nitrate into sulfuric acid, stirring for 1-2 hours, adding potassium permanganate, stirring for 1-2 hours, adding water, continuously stirring for 1-2 hours, heating and refluxing for 1-3 hours, adding hydrogen peroxide to stop reaction, washing, freeze-drying, grinding to obtain the oxidation graphene by mixing the oxidation graphene L-cysteine and boric acid obtained in the step one, adding deionized water, ultrasonic, adjusting pH to 8-9, transferring to the reaction vessel, hydrothermal reaction, cooling, washing, freeze-drying to obtain boron/sulfur/nitrogen-doped reducing oxidized graphene powder (BSN-rGO), dispersing the boron/sulfur/nitrogen-doped reducing oxidized graphene powder (BSN-rGO) obtained in step two in water, ultrasonic for 1-2 hours to form suspension, adding chloroauric acid (HAuCl4) solution and sodium citrate solution into the suspension, stirring for 0.5-1 hours at 15-35 degrees centigrade, transferring to the flask, microwave reaction, cooling, washing, drying to obtain gold-modified boron/sulfur/nitrogen-doped reduction graphene (AuBSN-rGO), uniformly dispersing the gold modified boron/sulfur/nitrogen-doped reduction graphene (Au-BSN-rGO) obtained in the step three in water to obtain dispersion, adding H2PtCl6 solution into the dispersion, then adding sodium borohydride, stirring at 15-35 degrees centigrade, reacting and washing, drying to obtain platinum/gold modified boron/sulfur/nitrogen-doped reduction graphene (Pt/Au-BSN-rGO). INDEPENDENT CLAIMS are included for: (1) a platinum/gold nano-particle modified boron/sulfur/nitrogen doped reducing oxidation graphene (Pt/Au-BSN-rGO), which obtains by the preparation method; and (2) a preparation method of electrochemical immune sensor, which involves: (a) coating the platinum/gold nano-particle modified boron/sulfur/nitrogen-doped reducing oxidation graphene the suspension on the Glassy carbon electrodes (GCE) surface, and drying sequentially the binding antibody, sealing agent and antigen, obtaining the electrochemical immune sensor.