▎ 摘　　要

NOVELTY - Preparation of dendritic nanocomposite doxorubicin electrochemical sensor involves mixing graphene oxide and carbon dots, obtaining carbon dot/graphene oxide homogeneous dispersion, adding mixed solution obtained by mixing silver nitrate aqueous solution and ammonium hydroxide aqueous solution, mixing, adding the resultant aqueous dispersion into an electrolytic cell containing phosphate buffered water, inserting a glassy carbon electrode into the cell, performing cyclic voltammetry, and depositing graphene oxide and silver nanoparticles at the electrode interface. USE - Preparation of dendritic nanocomposite electrochemical sensor used for detecting doxorubicin (claimed). ADVANTAGE - The method enables efficient dendritic nanocomposite electrochemical sensor by simple and economical process. The sensor enables efficient detection of doxorubicin with high sensitivity. DETAILED DESCRIPTION - Preparation of dendritic nanocomposite doxorubicin electrochemical sensor involves uniformly mixing graphene oxide and carbon dots, obtaining 1-2 mg/ml carbon dot/graphene oxide homogeneous dispersion, adding mixed solution obtained by mixing 50 mmol/L silver nitrate aqueous solution and 40 mmol/L ammonium hydroxide aqueous solution, mixing uniformly, adding the resultant aqueous dispersion into an electrolytic cell containing 1 mmol/L phosphate buffered water, inserting a glassy carbon electrode into the electrolytic cell, performing cyclic voltammetry at 0-50 mV/second, and 0-2 V, performing electrodeposition of graphene oxide and silver nanoparticles at the glassy carbon electrode interface, obtaining silver nanoparticle/carbon dot/reduced graphene oxide three-component dendritic nanocomposite at the glassy carbon electrode interface, drawing a electrochemical sensing curve using silver nanoparticle/carbon dots/reduced graphene oxide/glassy carbon electrode electrochemical sensing system as a sensor by differential pulse voltammetry, and calculating oxidation current peak intensities (Ip) (in mu A) based on the relation: Ip=2.197+8.293(DOX), where (DOX) is doxorubicin concentration of 0-250x 10-8 mol/L. The graphene oxide is prepared by adding 1 g graphite into 250 mL-round-bottom three-neck flask, adding 25 ml concentrated sulfuric acid, stirring at low speed, reacting for 24 hours, adding 1.5 g potassium permanganate, cooling in ice bath, stirring, reacting for 30 minutes, increasing the temperature to 60 degrees C, stirring, reacting for 45 minutes, adding 3 ml double-distilled water at each interval time of 15 minutes, adding 180 ml double distilled water, terminating the reaction, cooling the resultant product to room temperature, filtering, precipitating, washing, and drying. The carbon dots are prepared by dissolving 1 g citric acid and 2 g ammonium bicarbonate in 10 ml dimethyl sulfoxide, adding the resultant solution into a reactor, reacting at 160 degrees C for 6 hours, cooling the resultant reaction solution to room temperature, adding 20 mL 50 mu g/ml sodium hydroxide aqueous solution, stirring, reacting for 1 minute, centrifuging for 10 minutes in a centrifuge at 16000 rpm, washing the resultant precipitate using double distilled water, centrifuging and separating again to remove residual salt and alkali, and freeze-drying. The mass ratio of carbon dots and graphene oxide is 1:5-5:1. The mass ratio of carbon dots/graphene oxide, and mixed solution is 1:10-10:1.