• 专利标题:   Graphene quantum dot fluorescent probe material used as nuclear targeting imaging fluorescent probe for HeLa cells or other living cells, is zero-dimensional carbon nanomaterial at nanometer scale.
  • 专利号:   CN110205125-A
  • 发明人:   WANG L, LI M, LI W, GUO H, LIU Y
  • 专利权人:   UNIV SHANGHAI
  • 国际专利分类:   B82Y020/00, B82Y040/00, C09K011/65, G01N021/64
  • 专利详细信息:   CN110205125-A 06 Sep 2019 C09K-011/65 201977 Pages: 7 Chinese
  • 申请详细信息:   CN110205125-A CN10564370 27 Jun 2019
  • 优先权号:   CN10564370

▎ 摘  要

NOVELTY - Graphene quantum dot fluorescent probe material is a zero-dimensional carbon nanomaterial at a nanometer scale, and has a diameter of 2.2-6.7 nm and a thickness of 0.9-2.2 nm. The graphene quantum dots contain four elements including carbon, nitrogen, oxygen and hydrogen. USE - Graphene quantum dot fluorescent probe material used as nuclear targeting imaging fluorescent probe for HeLa cells or other living cells (all claimed). ADVANTAGE - The graphene quantum dot fluorescent probe material has good water solubility, good fluorescence stability, excellent cell imaging effects, and attractive application prospects in bioimaging technology, preferably in nuclear imaging. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for preparing graphene quantum dot fluorescent probe material, which involves: (A) dispersing 1,2,4-triaminobenzene dihydrochloride salt uniformly in an aqueous solution under ultrasonic conditions; (B) mixing into a mixed solution having a concentration of 1 to 10 mg/ml; (C) adding 1 M alkaline aqueous solution; (D) carrying out ultrasonic dispersion of the prepared solution for 10-120 minutes at 90-230 degrees C; (E) cooling at room temperature; (F) filtering through a microporous membrane having a pore diameter of 220 nm; and (G) transferring the filtered filtrate to a dialysis bag for dialysis for purifying to obtain graphene having nuclear targeted imaging. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic X-ray diffraction pattern of graphene quantum dots. (Drawing includes non-English language text).