• 专利标题:   Liquid biopsy for ovarian cancer based on graphene nano-sensor by e.g. collecting and detecting blood of patient, using improved Hummers method to prepare oxidation graphene, and adding buffer solution and graphene oxide solution to ovarian tissue to prepare solution for fluorescent measurement.
  • 专利号:   CN115896248-A
  • 发明人:   GUO Y, HAN L, CHEN Y, WAN Q, ZHENG A
  • 专利权人:   UNIV SICHUAN
  • 国际专利分类:   C12Q001/682, C12Q001/6825, G01N021/64
  • 专利详细信息:   CN115896248-A 04 Apr 2023 C12Q-001/682 202341 Chinese
  • 申请详细信息:   CN115896248-A CN11481717 24 Nov 2022
  • 优先权号:   CN11481717

▎ 摘  要

NOVELTY - Liquid biopsy for ovarian cancer based on graphene nano-ultrasonic sensor comprises (i) directly collecting and detecting the blood of the patient, (ii) using improved Hummers method to prepare the oxidation graphene, and characterizing the prepared oxidation graphene, and (iii) adding two different buffer solution and graphene oxide (GO) solution to ovarian tissue detected by puncture biopsy to prepare solution for fluorescent measurement. USE - The method is useful for liquid biopsy for ovarian cancer based on graphene nano-sensor. ADVANTAGE - The method realizes fluorescent biosensor for amplifying and detecting miRNA through graphene oxide and DSN enzymatic cleavage catalytic cycle, detects miRNA with high sensitivity, improves sensitivity, shortens reaction time, allows target miRNA molecule to cycle and hybridize with multiple DNA probe molecules, and amplifies fluorescent signal to achieve highly sensitive detection of miRNA.