▎ 摘　　要

NOVELTY - A three-dimensional reduced graphite oxide-chitosan-bovine serum albumin (3D-rGO-CS-BSA) compound modified electrode for electrochemical chiral recognition, comprises electrode and 3D-rGO-CS-BSA compound modified on the surface of the electrode. The modified electrode is obtained by dripping 3D-rGO-CS-BSA suspension on the surface of the electrode and drying. USE - Three-dimensional reduced graphite oxide-chitosan-bovine serum albumin compound modified electrode for electrochemical chiral recognition of 1-Boc-3-hydroxypyrrolidine enantiomer (claimed). ADVANTAGE - The 3D-rGO-CS-BSA compound modified electrode has simple and easy operation, high stability and good chiral recognition effect. The three-dimensional reduced graphene oxide has large specific surface area and improved conductivity. The open and interconnected porous structure of the framework provides an effective substrate for CS and BSA. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: preparation of 3D-rGO-CS-BSA composite modified electrode, which involves (S1) adding specific amount of graphite oxide (GO), dispersing in deionized water, ultrasonically treating, adding L-cysteine into solution, adding aqueous ammonia in drops, placing the mixture in oil bath for heating, cooling to room temperature, washing black product with deionized water, drying to obtain three-dimensional reduced graphite oxide (3D-rGO) powder, (S2) adding specific amount of 3D-rGO powder to prepare dispersion liquid, activating carboxylic acid groups in the 3D-rGO using 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide, dispersing carboxylic acid groups in the mixed solution of bovine serum albumin (BSA) and chitosan (CS), ultrasonically treating to obtain 3D-rGO-CS-BSA suspension, and (S3) using 3D-rGO-CS-BSA suspension, dripping on the surface of the electrode, and drying the electrode under infrared lamp; and application of 3D-rGO-CS-BSA complex modified electrode for electrochemical chiral recognition of 1-Boc-3-hydroxypyrrolidine enantiomer, which involves adding specific amount of R-/S-1-Boc-3-hydroxypyrrolidine, placing into volumetric flask, adding specific amount of prepared Phosphate-buffered saline (PBS), shaking to obtain enantiomer solution, soaking 3D-rGO-CS-BSA compound modified electrode into enantiomer solution, taking out, placing under infrared lamp for drying to obtain 1-Boc-3-hydroxypyrrolidine enantiomer electrode, using three-electrode system, using 1-Boc-3-hydroxypyrrolidine enantiomer electrode as working electrode, platinum sheet electrode as counter electrode and saturated calomel electrode as reference electrode, and immersing the three-electrode system into the prepared 0.1M potassium ferricyanide at room temperature and performing differential pulse test with potential range of -0.2-0.6 V.