• 专利标题:   Reduction of graphene oxide with microbial fermentation broth includes constructing Escherichia coli, inoculating Escherichia coli in fermentation medium, performing anaerobic fermentation on fermentation broth, filtering and sterilizing.
  • 专利号:   CN111620322-A
  • 发明人:   WANG Y, WANG X, YONG Y
  • 专利权人:   UNIV JIANGSU
  • 国际专利分类:   C01B032/184
  • 专利详细信息:   CN111620322-A 04 Sep 2020 C01B-032/184 202081 Pages: 14 Chinese
  • 申请详细信息:   CN111620322-A CN10471446 29 May 2020
  • 优先权号:   CN10471446

▎ 摘  要

NOVELTY - Reduction of graphene oxide with microbial fermentation broth comprises constructing Escherichia coli to express (FeFe) hydrogenase heterologously, inoculating Escherichia coli in fermentation medium, adding isopropyl beta -d-1-thiogalactopyranoside (IPTG) to induce expression of hydrogenase, performing anaerobic fermentation on fermentation broth in constant temperature shaker to obtain supernatant of fermentation broth, performing anaerobic centrifugation on supernatant of fermentation broth, filtering supernatant, sterilizing to obtain aseptic anaerobic fermentation broth, adding fermentation broth with graphene oxide aqueous solution and hydrogen under anaerobic conditions and culturing under constant temperature oscillation. USE - The method is for reduction of graphene oxide with microbial fermentation broth.