▎ 摘　　要

NOVELTY - The apparatus has a rigid base frame (12), and a base polymer sheet that is mounted to the base frame. The sheet is patterned with window (28), a bottom low water permeability barrier film, a top low water permeability barrier film, spacers separating the bottom low permeability barrier film from the top low permeability barrier film, and the spacers and barrier films forming chamber (26), an upper polymer sheet patterned with window, and an upper rigid frame. A target mounting support (14) is coupled with the rigid base frame. The mounting support is configured to fit in an X-ray or XFEL diffractometer. The input channels are fluidly coupled to one input port in the top ridged frame and to chamber. The output channels are fluidly coupled to chamber and to output port in top ridged frame. USE - Fixed target apparatus for serial x-ray crystallography. ADVANTAGE - The target is inexpensive, robust, rapidly fabricated polymer-based chips that maintain protein crystal hydration that allow on-chip crystallization as well as sample stability on chip for days to weeks. The target chips are optimized to minimize background by controlling the sample thickness in the beam to match the crystal size enabling fast raster scanning at lower fidelity and high hit rates that facilitate dynamic measurements. The use of amorphous, low-Z, thin flexible polymer films with low X-ray attenuation has enabled low water permeation rates and enduring hydration conditions. The polymer films can be chemically functionalized with binding groups to selectively nucleate and grow protein crystals in situ at desired locations with high yield. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for producing fixed target for serial x-ray crystallography. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic diagram of fixed target apparatus for serial x-ray crystallography. Target chip (10) Rigid base frame (12) Target mounting support (14) Chamber (26) Window (28)