▎ 摘　　要

NOVELTY - Magnetic immobilized enzyme reactor based on DNA tetrahedron comprises A magnetic oxidation graphene, gold nanoparticles immobilized on magnetic oxidation graphene, DNA tetrahedron modified on gold nanoparticles, and trypsin immobilized on DNA tetrahedron. USE - Magnetic immobilized enzyme reactor based on DNA tetrahedron used for protein fast enzyme cutting (claimed). ADVANTAGE - The magnetic immobilized enzyme reactor based on DNA tetrahedron is convenient for fast separating and recycling immobilized enzyme reactor, has large specific surface area, effectively improves solid loading amount of trypsin, realizes recycling of enzyme, avoids enzyme activity loss caused by self-digestion of enzyme, and improves repeated service life and enzymolysis efficiency. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: 1. a method for preparing magnetic immobilized enzyme reactor based on DNA tetrahedron, which involves: a. preparing magnetic oxidation graphene by solvent thermal method; b. loading gold nanoparticles fixedly on magnetic graphene oxide; c. synthesizing DNA tetrahedron with sulfydryl at top point by self-assembling reaction; d. modifying DNA tetrahedron on gold nanoparticle by covalent combination between gold and sulfur; and e. immobilizing covalent bond formed by bonding carboxyl and amino on DNA tetrahedron to obtain magnetic immobilized enzyme reactor; and 2. a method for using magnetic immobilized enzyme reactor based on DNA tetrahedron for protein fast enzyme cutting, which involves: a. mixing and incubating immobilized enzyme reactor and modified protein sample, after finishing enzyme cutting, separating magnetic material and supernatant by external magnet.