▎ 摘 要
NOVELTY - Label-free immunosensor comprises carbon electrodes, three-dimensional graphene-gold complex coupled between carbon electrodes, and three-dimensional graphene-gold complex coupled with prostate-specific antigen-monoclonal antibody, where the three-dimensional graphene-gold complex is formed by depositing gold nanoparticles with spherical shape on surface of several graphene. USE - The label-free immunosensor is useful for detecting prostate specific antigen (claimed). ADVANTAGE - The label-free immunosensor detects prostate specific antigen with improved selectivity, specificity and sensitivity. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method for producing the label-free immunosensor, which involves preparing a mixture comprising graphene oxide and gold precursor, performing aerosol spray pyrolysis method using the mixture to obtain three-dimensional graphene-gold complex, and coupling the three-dimensional graphene-gold complex on the carbon electrode and prostate-specific antigen-monoclonal antibody.