▎ 摘　　要

NOVELTY - The method involves forming a conductive metal layer (2) between a working electrode and a reference electrode on a micro-needle substrate (1) by magnetron sputtering method. A graphene composite material is reduced electrochemically on the conductivity metal layer of the working electrode under different conditions to form a transmission layer. A glucose oxidase layer is dropped on the corresponding transmission layer to obtain a glucose sensor, or a sodium ion sensitive layer is dropped on a corresponding transverse layer under special voltage to obtain the sodium ion sensor. The silver nano-layer is formed on the conductor of the conductive metals of the reference electrode, and the silver nano-layer is chloridized to obtain silver/silver chloride layer. USE - Method for preparing wearable micro-needle sensor for tissue fluid detection. Uses include but are not limited to blood sugar, lactic acid, sodium ion, content of potassium ion and pH and physiological electric signal in blood. ADVANTAGE - The wearable micro-needle sensor has high sensitivity, wide measuring range, simple manufacturing, and capable of pasting on the surface skin and quickly realizing tissue fluid detection. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic view of a method for tissue fluid detection of the wearable sensor. 1Micro-needle substrate 2Conductive metal layer 21Lead 22Electrode area