• 专利标题:   Method for preparing chitosan/ionic liquid-graphene composite-modified molecularly imprinted electrode i.e. utilized for detecting bovine serum albumin in plasma, involves preparing chitosan/ionic liquid-graphene-glassy carbon electrode.
  • 专利号:   CN103926294-A, CN103926294-B
  • 发明人:   CHI D, LI F, LI Y, XIA J, XIA L, XIA Y, ZHANG F, WANG Z, ZHAO K
  • 专利权人:   UNIV QINGDAO
  • 国际专利分类:   G01N027/327
  • 专利详细信息:   CN103926294-A 16 Jul 2014 G01N-027/327 201467 Pages: 11 Chinese
  • 申请详细信息:   CN103926294-A CN10167710 24 Apr 2014
  • 优先权号:   CN10167710

▎ 摘  要

NOVELTY - A chitosan/ionic liquid-graphene composite (CS/IL-GR)-modified molecularly imprinted electrode preparing method involves preparing chitosan/ionic liquid-graphene composite-glassy carbon electrode (DCS/IL-GR/GCE) by performing metallographical polishing process on a glassy carbon electrode by using alumina powder on a mirror surface. The electrode is rinsed with secondary water and washed with distilled water and ethanol ultrasonically. The electrode is dried, followed by dispersing 1-butyl-3-methylimidazolium hexafluorophosphate in aqueous solution of graphene ultrasonically. USE - Method for preparing chitosan/ionic liquid-graphene composite-modified molecularly imprinted electrode that is utilized for detecting bovine serum albumin in plasma (claimed). ADVANTAGE - The method enables preparing the electrode with better selectivity, sensitivity and reproducibility. DETAILED DESCRIPTION - A chitosan/ionic liquid-graphene composite (CS/IL-GR)-modified molecularly imprinted electrode preparing method involves preparing chitosan/ionic liquid-graphene composite-glassy carbon electrode (DCS/IL-GR/GCE) by performing metallographical polishing process on a glassy carbon electrode by using alumina powder with size of 0.3 mu m and 0.05 mu m on a mirror surface. The electrode is rinsed with secondary water and washed with distilled water and ethanol ultrasonically for 20 seconds. The electrode is dried, followed by dispersing 250 mu l 1-butyl-3-methylimidazolium hexafluorophosphate in aqueous solution of 1.0 mg/ml graphene ultrasonically to obtain ionic liquid-graphene composite (IL-GR) solution. The dried electrode is treated with 0.5 mu l IL-GR solution and dried to obtain IL-GR/GCE electrode, which is coated with 5.0 mu l solution containing 4 mass% chitosan and 2 vol.% acetic acid to obtain chitosan modified IL-GR/GCE electrode that is CS/IL-GR/GCE electrode. The CS/IL-GR/GCE electrode is immersed in 1.0 g/l bovine serum albumin and phosphate buffer containing oxygen and pyrrole monomer at potential range of -0.2 to 1.2 V and cyclic voltammetry scan rate of 100 mV/S to stabilize the electrode. The electrode is dried to obtain polymer modified CS/IL-GR/GCE electrode that is BSA-MIPs/CS/IL-GR/GCE electrode, which is immersed in imprinted template molecules and 1.0 M sulfuric acid solution to obtain MIPs/CS/IL-GR/GCE eelctrode as a finished product. An INDEPENDENT CLAIM is also included for a chitosan/ionic liquid-graphene composite-modified molecularly imprinted electrode.