▎ 摘　　要

NOVELTY - Detecting 1,5-anhydroglucitol based on composite material modified LAPS chip comprises e.g. preparing AuNPs by heating and stirring chloroauric acid until it boils, adding sodium citrate solution, stirring until the solution changes from light yellow to wine red, cooling to obtain AuNPs, preparing reduced graphene oxide (RGO) by weighing graphene oxide and placing it in ultrapure water, and using a cell disruptor to ultrasonically break it to obtain GO stock solution, using ascorbic acid for reduction under heating to obtain RGO, preparing chitosan-ferrocene by dissolving chitosan in acetic acid solution, adding ferrocene in formic acid, catalyzing with N-hydroxysuccinimide/1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (NHS/EDC) and stirring until the color of the solution turns reddish brown to obtain a chitosan-ferrocene (CS-Fc) solution, preparing RGO-CS-Fc by mixing RGO and CS-Fc solution, using NHS/EDC to catalyze, continuously heating, stirring and centrifuging. USE - The method is useful for detecting 1,5-anhydroglucitol based on composite material modified LAPS chip. ADVANTAGE - The method has highly fast and sensitive detection of 1,5AG. DETAILED DESCRIPTION - Detecting 1,5-anhydroglucitol based on composite material modified LAPS chip comprises (a) preparing AuNPs by heating and stirring chloroauric acid until it boils, adding sodium citrate solution, stirring until the solution changes from light yellow to wine red, cooling to obtain AuNPs, (b) preparing reduced graphene oxide (RGO) by weighing graphene oxide and placing it in ultrapure water, and using a cell disruptor to ultrasonically break it to obtain GO stock solution, using ascorbic acid for reduction under heating to obtain RGO, (3) preparing chitosan-ferrocene by dissolving chitosan in acetic acid solution, adding ferrocene in formic acid, catalyzing with N-hydroxysuccinimide/1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (NHS/EDC) and stirring until the color of the solution turns reddish brown to obtain a chitosan-ferrocene (CS-Fc) solution, (4) preparing RGO-CS-Fc by mixing RGO and CS-Fc solution, using NHS/EDC to catalyze, continuously heating, stirring, centrifuging and re-dissolving in ultra-pure water to obtain RGO-CS-Fc solution, modifying the LAPS sensor sensitive unit (a) orderly placing the light-addressable potentiometric sensor (LAPS) chip in ethanol and acetone solution, using ultrasonic cleaning instrument for ultrasonic cleaning, washing with pure water, drying the electrode surface, (b) dripping sodium hydroxide activation on the surface of the LAPS chip, washing by pure water, drying the surface of the LAPS chip, (c) carrying out silanization treatment to the surface of the activated LAPS chip, using natural sedimentation method, depositing the nano-gold on the LAPS chip after silanization, (5) on the LAPS chip deposited gold nanoparticle (AuNPs), dropping RGO-CS-Fc suspension, drying, constructing the LAPS sensor detection system through glutaraldehyde 7-pentoxyresorufin (PROD) enzyme cross-linked to the LAPS chip surface to obtain the LAPS biosensor with PROD/RGO-CS-Fc/Au NPs/SiO2-Si composite structure, mixing the LAPS biosensor, data collecting card, LabVIEW (RTM: Data collecting software), laser diode and so on to form LAPS biosensor detection system, drawing of 5-AG standard curve, dropping 1, 5-AG standard solution with different concentrations on the surface of the LAPS chip, incubating, washing with phosphate buffered saline solution, using the data collecting card and LabVIEW (RTM: Data collecting software) the light current and bias voltage data output by the LAPS detection system, processing and analyzing, drawing the standard curve, calculating the lowest detection limit of the method in the actual sample 1, detecting the 5-AG sample to be tested, adding to the LAPS biosensor interface obtained in step for incubation, washing by phosphate buffered saline solution, dropping phosphate buffered saline solution on the modified LAPS chip, using data collecting card and LabVIEW (RTM: Data collecting software) the light current and bias voltage data output by the LAPS detection system, processing and analyzing, according to the standard curve and calculating to obtain the concentration of 1, 5-AG in the actual sample to be tested.