• 专利标题:   Preparing cell culture carrier capable of regulating growth state of cells, comprises preparing large-size graphene oxide dispersion, preparing graphite oxide according to Hummers method or modified Hummers method.
  • 专利号:   CN113184839-A, CN113184839-B
  • 发明人:   MA Y, LI S, JI H, WANG J, WANG Q
  • 专利权人:   UNIV SHENYANG JIANZHU
  • 国际专利分类:   C01B032/192, C12N005/00, C12N005/077
  • 专利详细信息:   CN113184839-A 30 Jul 2021 C01B-032/192 202180 Pages: 10 Chinese
  • 申请详细信息:   CN113184839-A CN10515431 12 May 2021
  • 优先权号:   CN10515431

▎ 摘  要

NOVELTY - Preparing a cell culture carrier capable of regulating the growth state of cells, comprises preparing a large-size graphene oxide dispersion, preparing graphite oxide according to the Hummers method or the modified Hummers method, and reducing residual oxidant with hydrogen peroxide, letting stand to remove the lower layer, washing with deionized water and dilute hydro chloric acid solution respectively, and dialysis to neutral to prepare a large-size graphene oxide dispersion. The concentration of the size graphene oxide dispersion liquid is prepared into graphene oxide ink, the graphene oxide ink is printed on the substrate, and after freezing, it is vacuum freeze-dried in a freeze dryer for 6-10 hours to obtain graphene oxide aerogel. The graphene oxide aerogel is chemically reduced, after the reduction, it is taken out and naturally cooled, washed to neutrality, and dried to obtain the graphene aerogel. The substrate with the graphene aerogel attached is the prepared material. USE - Method for preparing a cell culture carrier capable of regulating the growth state of cells. ADVANTAGE - The method enables to prepare cell culture carrier capable of regulating the growth state of cells. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for utilizing the cell culture carrier capable of regulating cell growth, which comprises (A) removing the frozen cells from liquid nitrogen, fixing in a 37 degrees C water bath for cell recovery, removing the cells before they are completely thawed, adding the cell culture solution and centrifuging, taking the bottom cell sediment, shaking well, adding the cell culture solution and transferring it into the culture flask, and placing it in the cell culture box for culture; and (B) transferring the culture solution and the cultured cells into a cell culture dish equipped with the cell culture carrier capable of regulating the growth state of the cells, and allowing the cells to grow on the culture carrier.