▎ 摘　　要

NOVELTY - Preparing biosensor comprises (i) drop-coating Trp-AuNPs-IL-rGO suspension on clean glassy carbon electrode (GCE), and drying to obtain Trp-AuNPs-ILrGO/GCE, where the Trp-AuNPs-IL-rGO includes amino-ionic graphene and gold nanoparticles loaded on it with tryptophan reduced and wrapped, and (ii) using 1-ethyl-3-(3-dimethylaminopropyl)-1-carbodiimide hydrochloride/N-hydroxysuccinimide solution to active Trp-AuNPs-IL-rGO/GCE, and immersing Trp-AuNPs-IL-rGO/GCE in ethylenediamine solution to block carboxy groups, using antibody to modify Trp-AuNPs-IL-rGO/GCE, fixing carboxy group of Fc bottom and Fab shoulder of antibody AbGRα to amino group of Trp-AuNPs-IL-rGO/GCE during the modification process, using bovine serum albumin to remove non-specific binding sites to seal electrode surface, and washing electrode with phosphate buffered saline buffer solution to remove unattached buffered saline buffer on electrode surface to obtain biosensor BSA-AbGRα-Trp-AuNPs-ILrGO/GCE. USE - The method is useful for preparing biosensor for detecting glucocorticoid receptor alpha protein content and evaluating depression markers (all claimed). ADVANTAGE - The biosensor has 0.283 pg/ml limit of detection (LOD), good reproducibility, long-term stability and specificity. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: use of biosensor in detecting GRα protein content. evaluating depression markers, comprising (i) immerse bovine serum albumin-AbGRα-Trp-AuNPs-ILrGO/GCE in different concentrations of GRα protein standard solutions and incubating, completing incubation, testing and establishing concentration-DPV standard curve, (ii) immersing bovine serum albumin-AbGRα-Trp-AuNPs-IL-rGO/GCE in GRα protein solution to be tested for incubation, and performing DPV test after incubation, and substituting obtained test results into standard curve obtained in step (i) to calculate concentration of GRα protein solution to be tested.