▎ 摘　　要

NOVELTY - Preparing injectable hydrogel wound dressing with tissue adhesion, multiple sterilization and electrical stimulation tissue regeneration functions comprises (i) stirring graphene oxide in deionized water, adding phytic acid, adding aniline monomer, dripping initiator solution, preparing highly dispersed graphene oxide/polyaniline nanofillers by in-situ free radical polymerization of aniline on surface of graphene oxide, (ii) dispersing graphene oxide/polyaniline nanofiller in 2 mg/ml DA and 10 mM Tris buffer solution, and obtaining graphene oxide/polyaniline/polydopamine nanofillers, (iii) preparing a silver nitrate solution under light-shielding conditions, and preparing oxide/polyaniline/polydopamine-nano silver nanofiller, (iv) dissolving gelatin methacryloyl (GelMA) in phosphate-buffered saline, adding quaternized chitosan, catechol-modified chitosan, and graphene oxide/polyaniline/polydopamine-nano-silver nano-filler, and (v) injecting mixed solution into wound site. USE - The method is useful for preparing injectable hydrogel wound dressing with tissue adhesion, multiple sterilization and electrical stimulation tissue regeneration functions. ADVANTAGE - The material: has excellent wet tissue adhesion, electrical conductivity, antibacterial properties, injectability and biocompatibility; and can be used as a new type of wound dressing to treat various irregular wounds. DETAILED DESCRIPTION - Preparing injectable hydrogel wound dressing with tissue adhesion, multiple sterilization and electrical stimulation tissue regeneration functions comprises (i) firstly taking graphene oxide and stirring in deionized water, uniformly and ultrasonically dispersing, adding phytic acid, dispersing under triple process of vigorous stirring, vortex and ultrasonic, adding aniline monomer, continuously dispersing under triple process of vigorous stirring, vortex and ultrasonic, finally dripping initiator solution, preparing highly dispersed graphene oxide/polyaniline nanofillers by in-situ free radical polymerization of aniline on surface of graphene oxide under vigorous stirring conditions, washing and freeze-drying, (ii) ultrasonically dispersing graphene oxide/polyaniline nanofiller prepared in the step (i) in 2 mg/ml DA and 10 mM Tris buffer solution (pH = 8.5), reacting for 2-6 hours to obtain graphene oxide/polyaniline/polydopamine nanofillers, (iii) preparing a silver nitrate solution under light-shielding conditions, placing graphene oxide/polyaniline/polydopamine nanofiller obtained in the step (ii) in the above solution and reacting for 12-24 hours to prepare graphene oxide/polyaniline/polydopamine-nano silver nanofiller, (iv) dissolving specific amount of gelatin methacryloyl (GelMA) in phosphate-buffered saline at 60°C to obtain a uniform solution, cooling to 30°C, adding quaternized chitosan, catechol-modified chitosan, and graphene oxide/polyaniline/polydopamine-nano-silver nano-filler prepared in step (iii) and de-aerating by low-speed centrifugation, and (v) using injection device to inject mixed solution prepared in the step (iv) into wound site, and performing in-situ solidifying to obtain hydrogel dressing of required shape to realize accurate coverage of the wound. An INDEPENDENT CLAIM is also included for an injectable hydrogel wound dressing with tissue adhesion, multiple sterilization and electrical stimulation tissue regeneration functions prepared by the above-mentioned method.